Lignocellulosic Cellulases

Bioresource technology 101 (2010) 87988806 Contents lists available at ScienceDirect Bioresource Technology diary homepage: www.elsevier.com/locate/biortech Characterization of thermostable cellulases produced by Bacillus and Geobacillus ancestrys Gurdeep Rastogi a,1, Aditya Bhalla a, Akash Adhikari a, Kenneth M. Bischoff b, Stephen R. Hughes b, Lew P. Christopher c, Rajesh K. Sani a,* a b c Department of Chemical and Biological Engineering, reciprocal ohm Dakota School of Mines and Technology, rapid City, SD 57701, regular army Bioproducts and Biocatalysis explore Unit, National spunk for Agricultural function Research, US Department of Agriculture, Peoria, IL 61604, the States Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA a r t i c l e i n f o a b s t r a c t The composition of thermophilic (60 °C) mixed cellulose-degrading enrichment culture initiated from convert sample s was examined by constructing a 16S rRNA gene clone library and the presence of sequences relate to to Actinobacteria, Bacteroidetes, Chloro?exi, Deinococcus-Thermus, Firmicutes, and Proteobacteria were identi?ed.

Eight secludes capable of degrading cellulose, carboxymethyl cellulose (CMC), or ponderosa true pine away sawdust were identi?ed as belonging to the genera Geobacillus, Thermobacillus, Cohnella, and Thermus. A compost isolate WSUCF1 (Geobacillus sp.) was selected based on its higher growth wander and cellulase natural action compared to others in liquid minimal culture medium containing cellulose as a source of carbon and energy. Strain WSUCF! 1 and a previously stray thermophilic cellulose-degrading deep gold mine strain DUSELR13 (Bacillus sp.) were examined for their enzyme properties and kinetics. The optimal pH for carboxymethyl cellulase (CMCase) activity was 5.0 for both isolates. The optimum temperatures for CMCase of WSUCFI and DUSELR13 were 70 and 75 °C, respectively. For CMC, the DUSELR13 and WSUCF1 CMCases had Km values...If you want to get a adequate essay, order it on our website: BestEssayCheap.com

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