Microbiology Lab

Unknown Final report 1. Introduction: The purpose of this riddleing ground was to spot two unfathomable bacterium cultures using confused derivative bear witnesss.   The identification of these isolated cultures was accomplished by separating and differentiating possible bacterium based on specialized biochemical characteristics.   Whether the mental testings performed determine specific enzymatic reactions or metabolic pathways, each(prenominal) was use in a way to help descry those specifics and identify the unknown cultures.   The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and saccharose fermentation, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, indole, urease, DNase, and coagulase.   The tests performed on the unknown bacteria cultures were all used to determine the individualism of the bacteria.   Each of the tests performed provided most key information about the bacteria in head word and how it functions.   All of the tests were performed on each culture, however, as some of the tests were used only for gram (+) or gram (-) bacteria, while others were nevertheless more specific and identified only for cocci bacteria.   The tests performed and what constitutes a positive and negative test are as follows. 2.

tryout Result of all existence similar to my unknown enter following(a) pages 3. Flow chart See page with flow chart 4. Test Result Oxidase This exam ground test is based on detecting the toil of the enzyme cytochrome oxidase by Gr am-negative bacteria if no color stir to p! urple or blue is evident at 30 seconds-1 slight then the result is positive. It is important that the test is evince by one minute to ensure accurate results (avoid trumped-up(prenominal) negatives and off-key positives).  Unknown 12 resulted in a negative test with no color change. These were similar results seen in Eschericia coli, Proteus vulgaris, genus genus Serratia marcescens, and Enterobacter aerogenes. This test helped to rule out two of the six unknown...If you sine qua non to get a full essay, order it on our website: BestEssayCheap.com

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